Review



evergreen dye  (Biotium)


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    Structured Review

    Biotium evergreen dye
    Evergreen Dye, supplied by Biotium, used in various techniques. Bioz Stars score: 97/100, based on 3286 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/evergreen+dye/EvaGreen/bio_rxiv__64898__2026__02__26__708234-196-19-21
    Average 97 stars, based on 3286 article reviews
    evergreen dye - by Bioz Stars, 2026-11
    97/100 stars

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    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: Identifying severe COVID-19 risk variants modulating enhancer reporter activity in lung cells
    Article Snippet: 15-bp flanking adapters with primer binding sites for PCR amplification were added (FW: ACGCTCTTCCGATCT, RV: GTGCTCTTCCGATCT) and the resulting STARR-seq library consisting of 13,802x 200-bp sequences synthesised as pooled oligonucleotides from Twist BioSciences (USA). .. The optimal number of cycles was determined by a qPCR test reaction using the same conditions, except 0.25 μl Evergreen dye (Biotium #31000-T) was added to the reaction and 30 cycles performed followed by a plate read step. .. Briefly, the protocol involved candidate sequence PCR amplification, digestion of the hSTARR screening vector (Addgene #99296), cloning of library inserts into the digested hSTARR vector and plasmid library amplification and purification.



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